human microvascular endothelial cell line  (ATCC)

Journal: Archives of Toxicology

Article Title: Okadaic acid triggers NFκB and STAT3 phosphorylation followed by a release of inflammatory markers in human and mouse endothelial cells

doi: 10.1007/s00204-026-04320-3

Figure Lengend Snippet: Effect of OA on viability and LDH release in human (HMEC-1) and mouse (MS1) microvascular endothelial cells. a Cell viability of HMEC-1 cells treated with OA for 3, 6–24 h. b Cell viability of MS1 cells treated with OA for 3, 6–24 h. Results are expressed as percentage of control cells and presented as mean ± SEM of three independent replicates performed by triplicate. c LDH release in HMEC-1 cells and d) MS1 cells treated with OA for 3, 6–24 h. Data are expressed as percentage of cell death control and presented as mean ± SEM of three independent replicates performed by duplicate. Statistical differences were assessed by one-way ANOVA followed by Dunnett’s tests (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 compared to untreated control).

Article Snippet: Human dermal microvascular endothelial cell line (HMEC-1) was obtained from American Type Culture Collection (ATCC), number CRL 3243.

Techniques: Control

Journal: Archives of Toxicology

Article Title: Okadaic acid triggers NFκB and STAT3 phosphorylation followed by a release of inflammatory markers in human and mouse endothelial cells

doi: 10.1007/s00204-026-04320-3

Figure Lengend Snippet: Measurement of IL-6 and CD147 release from human (HMEC-1) and mouse (MS1) microvascular endothelial cells treated with OA. a IL-6 release in HMEC-1 and b IL-6 release in MS1 after OA treatment. c Levels of CD147 receptor measured in HMEC-1. d Levels of CD147 receptor measured in MS1. LPS at 500 ng/ml was used as a positive control. Mean ± SEM of three independent replicates performed by duplicate. Statistical differences were assessed by one-way ANOVA followed by Dunnett’s tests (* p < 0.05, ** p < 0.01, *** p < 0.001 **** p < 0.0001 compared to untreated control.

Article Snippet: Human dermal microvascular endothelial cell line (HMEC-1) was obtained from American Type Culture Collection (ATCC), number CRL 3243.

Techniques: Positive Control, Control

Journal: Archives of Toxicology

Article Title: Okadaic acid triggers NFκB and STAT3 phosphorylation followed by a release of inflammatory markers in human and mouse endothelial cells

doi: 10.1007/s00204-026-04320-3

Figure Lengend Snippet: Analysis of NFκB and STAT3 activation in human (HMEC-1) and mouse (MS1) microvascular endothelial cells after treatment with OA. a Activation of NFκB-p65 in HMEC-1 cells and b activation of NFκB-p65 in MS1 cells. c Activation of STAT3 in HMEC-1 and d activation of STAT3 in MS1 cells. Data are present as ratio of phosphorylated and total protein and normalized by actin. Cells were incubated with 100 nM OA for 3 and 6 h. LPS 500 ng/mL was used as positive control. Mean ± SEM from three independent replicates performed in duplicate. Statistical differences were assessed by one-way ANOVA followed by Dunnett’s tests (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 compared to untreated control).

Article Snippet: Human dermal microvascular endothelial cell line (HMEC-1) was obtained from American Type Culture Collection (ATCC), number CRL 3243.

Techniques: Activation Assay, Incubation, Positive Control, Control